Journal: Cancer Science

Article Title: Copy number gain of ACTN4 is associated with poor prognosis in patients with upper urinary tract urothelial carcinoma

doi: 10.1111/cas.15841

Figure Lengend Snippet: Representative findings of fluorescence in situ hybridization for α‐actinin4 (ACTN4). Red and green signals indicate the ACTN4 and CEN19p signals, respectively. The figure shows the 4,6‐diamidino‐2‐phenylindone‐counterstained interphase nuclei for each specimen. (A) A case showing amplification as well as a higher copy number of ACTN4 . (B) A case showing increased numbers of both red and green signals, indicating a higher copy number but no amplification of ACTN4 (original magnification ×1000).

Article Snippet: ACTN4 SpectrumOrange/Chromosome 19 DNA probe ( CEN19p ) SpectrumGreen DNA probes (Abnova Corporation) were used for FISH analysis according to the same method as that of the PathVysion DNA probe kit (Abbott Molecular), as described previously.

Techniques: Fluorescence, In Situ Hybridization, Amplification

Journal: Cancer Science

Article Title: Copy number gain of ACTN4 is associated with poor prognosis in patients with upper urinary tract urothelial carcinoma

doi: 10.1111/cas.15841

Figure Lengend Snippet: Relationship between clinicopathological parameters and copy number gain of ACTN4 .

Article Snippet: ACTN4 SpectrumOrange/Chromosome 19 DNA probe ( CEN19p ) SpectrumGreen DNA probes (Abnova Corporation) were used for FISH analysis according to the same method as that of the PathVysion DNA probe kit (Abbott Molecular), as described previously.

Techniques: Dissection, Over Expression

Journal: Cancer Science

Article Title: Copy number gain of ACTN4 is associated with poor prognosis in patients with upper urinary tract urothelial carcinoma

doi: 10.1111/cas.15841

Figure Lengend Snippet: Kaplan–Meier estimates of (A) extraurothelial recurrence‐free survival; and (B) overall survival for all 168 patients stratified according to the status of copy number gain of α‐actinin4 ( ACTN4 ). Dashed line curves for cases with copy number gain of ACTN4 ( n = 25), and solid line curves for cases without copy number gain of ACTN4 ( n = 143). The two curves are statistically different [ p < 0.0001 in both (A, B)].

Article Snippet: ACTN4 SpectrumOrange/Chromosome 19 DNA probe ( CEN19p ) SpectrumGreen DNA probes (Abnova Corporation) were used for FISH analysis according to the same method as that of the PathVysion DNA probe kit (Abbott Molecular), as described previously.

Techniques:

Journal: Cancer Science

Article Title: Copy number gain of ACTN4 is associated with poor prognosis in patients with upper urinary tract urothelial carcinoma

doi: 10.1111/cas.15841

Figure Lengend Snippet: Univariate analysis for predicting EUR‐FS in patients with UUTUC.

Article Snippet: ACTN4 SpectrumOrange/Chromosome 19 DNA probe ( CEN19p ) SpectrumGreen DNA probes (Abnova Corporation) were used for FISH analysis according to the same method as that of the PathVysion DNA probe kit (Abbott Molecular), as described previously.

Techniques: Significance Assay, Dissection, Over Expression

Journal: Cancer Science

Article Title: Copy number gain of ACTN4 is associated with poor prognosis in patients with upper urinary tract urothelial carcinoma

doi: 10.1111/cas.15841

Figure Lengend Snippet: Multivariate analysis for predicting EUR‐FS in patients with UUTUC.

Article Snippet: ACTN4 SpectrumOrange/Chromosome 19 DNA probe ( CEN19p ) SpectrumGreen DNA probes (Abnova Corporation) were used for FISH analysis according to the same method as that of the PathVysion DNA probe kit (Abbott Molecular), as described previously.

Techniques:

Journal: Cancer Science

Article Title: Copy number gain of ACTN4 is associated with poor prognosis in patients with upper urinary tract urothelial carcinoma

doi: 10.1111/cas.15841

Figure Lengend Snippet: Kaplan–Meier estimates of (A) extraurothelial recurrence‐free survival; and (B) overall survival for all 168 patients stratified according to the status of α‐actinin4 (ACTN4) protein overexpression. Dashed line curves for cases with ACTN4 protein overexpression ( n = 49), and solid line curves for cases without ACTN4 protein overexpression ( n = 119). The two curves are statistically different [ p < 0.0001 in both (A, B)].

Article Snippet: ACTN4 SpectrumOrange/Chromosome 19 DNA probe ( CEN19p ) SpectrumGreen DNA probes (Abnova Corporation) were used for FISH analysis according to the same method as that of the PathVysion DNA probe kit (Abbott Molecular), as described previously.

Techniques: Over Expression

Journal: Cancer Science

Article Title: Copy number gain of ACTN4 is associated with poor prognosis in patients with upper urinary tract urothelial carcinoma

doi: 10.1111/cas.15841

Figure Lengend Snippet: Univariate analysis for predicting overall survival in patients with UTUUC.

Article Snippet: ACTN4 SpectrumOrange/Chromosome 19 DNA probe ( CEN19p ) SpectrumGreen DNA probes (Abnova Corporation) were used for FISH analysis according to the same method as that of the PathVysion DNA probe kit (Abbott Molecular), as described previously.

Techniques: Dissection, Over Expression

Journal: Cancer Science

Article Title: Copy number gain of ACTN4 is associated with poor prognosis in patients with upper urinary tract urothelial carcinoma

doi: 10.1111/cas.15841

Figure Lengend Snippet: Multivariate analysis for predicting overall survival in patients with UUTUC.

Article Snippet: ACTN4 SpectrumOrange/Chromosome 19 DNA probe ( CEN19p ) SpectrumGreen DNA probes (Abnova Corporation) were used for FISH analysis according to the same method as that of the PathVysion DNA probe kit (Abbott Molecular), as described previously.

Techniques: